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Santiago de los Treinta Caballeros is located on a hilly terrain in the middle of the Cibao Valley in the Central Region of the Dominican Republic, one of the most fertile lands found in the island. The Yaque del Norte river passes by Santiago which is in between Cordillera Central and Cordillera Septentrional, two of the three major mountain ranges on the island of Hispaniola, forming the Cibao Valley.